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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Deficiency of macrophage migration inhibitory factor attenuates tau hyperphosphorylation in mouse models of Alzheimer’s disease

Fig. 3

Staining of phosphorylated tau in brain slices from WT and MIF deficient mice. a, b Frozen slices from cortex and three different regions of hippocampus were stained for phosphorylated tau using rabbit polyclonal anti-Tau (pT205) and anti-Tau (pS396) antibodies. The sections were further treated with Alexa Fluor 568-conjugated anti-rabbit IgG (red fluorescence) and examined under a laser-scanning confocal microscope. Nuclei were stained with DAPI (blue). Scale bar in the upper left panes 75 μm (×200 magnification). Quantification of the level of tau phosphorylation at pT205 (c) and pS396 (d) in each of the regions, showing differences in fluorescence intensity between WT and Mif −/− mice receiving STZ and saline. Relative immunofluorescence was measured, and the results are presented as the means ± SEM based on five viewing fields for each region, using at least two mice. *p < 0.05, **p < 0.01 compared with Mif +/+ mice receiving saline

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