Fig. 3
WNV-specific IgM and IgG titers, neutralizing antibodies titers, and levels of IFN-α and IFN-β in mice following WNV NY99 and WNV Eg101 infection. Serum was collected from WNV NY99 and WNV Eg101 infected mice at indicated time points and WNV-specific IgM (a) and IgG (b) responses were measured by MIA using WNV E antigen. Results are reported as median fluorescent intensity (MFI) per 100 microspheres. Data are expressed as MFI ± SEM and is representative of two independent experiments (n = 8–16 mice per group). *p < 0.05, **p < 0.001. Horizontal dotted line indicates the cutoff value. c Neutralizing antibodies titers in the serum of WNV NY99 and WNV Eg101 infected mice. Serum collected from WNV NY99 and WNV Eg101 infected mice at indicated time points was serially diluted from 1:40 to 1:5000 and PRNT was conducted against WNV NY99 and WNV Eg101 viruses, respectively. Data are expressed as mean log10 titer ± SEM and is representative of two independent experiments (n = 8–16 mice per group). IFN-α and IFN-β production was measured in the serum (d) and (e) collected from WNV NY99 and WNV Eg101 infected mice at indicated time points using mouse IFN-α and mouse IFN-β ELISA kits and from brains (f) and (g) harvested from WNV NY99 and WNV Eg101 infected mice at days 6 and 8 after inoculation. The data are expressed as pg/mL of serum or pg/g of brain tissue. Error bars represent SEM (n = 6–8 mice per group). *p < 0.05, **p < 0.001