Fig. 4

PREP is expressed in neurons and astrocytes in hippocampus and cerebellum of PCS rats. PREP (red) is expressed mainly in the neurons of the CA1 region of the hippocampus visualized with NeuN (green) (a). High magnification images showed co-localization (arrows) of PREP (red) with astrocytes stained with glial fibrillary acidic protein (GFAP; green) in the hippocampus (b–d), in the white matter of the cerebellum (e–g) and the cerebellar cortex (h–j). To note is the strong PREP expression in Purkinje cells (arrow heads in (h, j)) also co-localization with the glia Bergman (arrows). Nuclear marker: DAPI (cyan). Scale bar: b–g 10 μm; a, h–j 30 μm