Fig. 2

Axonal injury is observed in various regions throughout the micro pig brain following cFPI. Representative photomicrographs of APP immunohistochemistry in regions of the micro pig brain that demonstrated DAI in animals sustaining cFPI. Images in the middle panel (b, f, i, l, o, r) are magnified regions indicated in the images of the left panel (a, e, h, k, n, q) and images in the right panel (c, g, j, m, p, s) are magnified regions indicated in the middle panel (b, f, i, l, o, r), respectively. Note that DAI within the thalamus and tectum was diffusely distributed throughout the domain, while DAI within the other regions was more localized. Also note that while not common, APP+ proximal axonal swellings in continuity with the neuronal soma (d) were observed in the thalamus. Scale bar in q: 200 μm; r and s: 100 μm; d: 50 μm