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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pig

Fig. 5

Extensive microglial activation is observed in the micro pig thalamus 6 h following diffuse mTBI. Representative photomicrographs of the microglial marker Iba-1 in the thalamus of sham-injured (ad) or central fluid percussion injured (eh) micro pigs. b and f are magnified regions indicated in a and e, and c and d are magnified regions indicated in b and f, respectively. d and h are two-dimensional flattened images of three-dimensional stacks through microglia in the sham (d) or injured thalamus (h). Note that the microglia appear ramified in sham-injured animals, indicating a quiescent state. While ramified microglia are present in brain-injured pigs, a large proportion of microglia have retracted, amoeboid or stellate morphologies, indicating activation. Bar graphs illustrating the degree of microglial activation in the thalamus (i) as well as the average soma size (j), average process length (k), average process number (l), average number of primary processes (m), and average number of end points (n) per Iba-1+ cell. Scale bar a and e = 1 mm, b and f = 200 μm, c and g = 40 μm, d and h = 10 μm. Graph depicts the mean ± standard error of the mean. *p < 0.05

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