Fig. 5

HSF1 inhibition fails to affect LPS-induced IκB-α degradation, NF-κB phosphorylation, and NF-κB nuclear translocation. a As shown, LPS-induced increases in IκB-α degradation and NF-κB phosphorylation level were not affected by KRIBB11 treatment (3 μM) in BV-2 cells (n = 3). b, c Quantitative analysis of the effects of KRIBB11 on IκB-α degradation (b) and NF-κB phosphorylation (c) in LPS-treated cells (n = 3, *P < 0.05, **P < 0.01 vs. control or KRIBB11 alone-treated group). d Representative images showing the effect of KRIBB11 on NF-κB nuclear translocation. Cells were stimulated with LPS for 30 min, and the levels of NF-κB in the cytoplasm and nucleus were detected. In KRIBB11-treated groups, cells were incubated with KRIBB11 for 30 min prior to LPS stimulation. e, f Quantitative analysis of NF-κB expression in the cytoplasm (e) and nucleus (f) in LPS-stimulated cells after KRIBB11 administration (n = 3, *P < 0.05 vs. control or KRIBB11 alone-treated group, **P < 0.01 vs. KRIBB11 alone-treated group). All data were shown as mean ± S.E. NS, no significance