Fig. 6

HSF1 inhibition does not inhibit STAT-1 phosphorylation and nuclear translocation in IFN-γ-stimulated cells. a As shown, KRIBB11 treatment did not attenuate the increase in STAT1 phosphorylation level in IFN-γ-stimulated BV-2 cells. b Quantitative analysis of the effect of KRIBB11 on STAT-1 phosphorylation in IFN-γ-treated cells (n = 3, *P < 0.05, **P < 0.01 vs. control group). c Representative images showing the effect of KRIBB11 on STAT-1 nuclear translocation. Cells were stimulated with IFN-γ for 30 min and the levels of STAT-1 in the cytoplasm and nucleus were detected. In KRIBB11-treated groups, cells were incubated with KRIBB11 for 30 min prior to IFN-γ stimulation. d, e Quantitative analysis of STAT-1 expression in the cytoplasm (d) and nucleus (e) in IFN-γ-stimulated cells after KRIBB11 administration (n = 3, *P < 0.05 vs. control or IFN-γ alone-treated group, **P < 0.01 vs. IFN-γ alone-treated group). All data were shown as mean ± S.E