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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Requirement for endogenous heat shock factor 1 in inducible nitric oxide synthase induction in murine microglia

Fig. 7

HSF1 inhibition attenuates the binding of active NF-κB and STAT-1 to their DNA elements in iNOS promoters. a Quantitative analysis of the effect of KRIBB11 on NF-κB binding to its DNA element. BV-2 cells were stimulated with LPS, and the binding activity of NF-κB p65 was measured in the absence or presence of KRIBB11 (n = 5, **P < 0.01 vs. LPS alone-treated group). b Quantitative analysis of the effect of KRIBB11 on p-STAT-1 binding to its DNA element in iNOS promoters. Cells were stimulated with IFN-γ, and the binding activity of p-STAT-1 was measured in the absence or presence of KRIBB11 (n = 5, **P < 0.01 vs. LPS alone-treated group). c Representative ChIP data showing that KRIBB11 markedly reduced NF-κB binding to the iNOS promoter in LPS-stimulated cells. The chromatin was immunoprecipitated using the anti-p65 antibody. d Quantitative analysis of the effect of KRIBB11 on the binding of NF-κB to the iNOS promoter in LPS-stimulated cells (n = 3, **P < 0.01 vs. control LPS alone-, or KRIBB11 alone-treated group). e Representative ChIP data showing that KRIBB11 markedly reduced p-STAT1 binding to the iNOS promoter in IFN-γ-stimulated cells. f Quantitative analysis of the effect of KRIBB11 on the binding of p-STAT1 to the iNOS promoter in IFN-γ-stimulated cells (n = 3, *P < 0.05 vs. LPS or KRIBB11 alone-treated group, **P < 0.01 vs. control group). All data were shown as mean ± S.E

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