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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Endogenous adaptation to low oxygen modulates T-cell regulatory pathways in EAE

Fig. 2

Chronic mild hypoxia delayed onset of EAE and caused less tissue hypoxia. Mice were immunized with MOG35-55 peptide+ CFA. Immediately following immunization, one group of animals was placed in the normobaric hypoxia chambers. The EAE-only group was left in the normoxic environment all the time. Clinical scores were determined daily. a The graph presents the mean ± SD of scores (n = 12 mice/group) including the score 0 from two independent experiments. Results confirm previous studies using smaller numbers of animals. The two-way ANOVA analysis revealed that the difference was statistically significant (p < 0.001). Tissue hypoxia was determined in spleens and spinal cords of all animals with pimonidazole (green) and Hoechst (blue, for nuclei) as described in the “Materials and methods” section. b Spleens of control and hypoxic mice at different time points following 10 % O2 treatment are representative of three mice/group/time point. Few positive cells (arrow) were detected only at the 24-h post-hypoxia group. Spinal cords at the peak disease (c) and spleens (d) after 1 and 2 weeks of EAE induction (n = 3/group). Magnification is ×40

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