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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Endogenous adaptation to low oxygen modulates T-cell regulatory pathways in EAE

Fig. 6

Hypoxia delayed the sensitization of IL-17A-producing T cells and modulated the inflammatory mediators. Spleens were removed from either hypoxic or normoxic MOG-immunized or control mice at days 7, 14, 23, and 41 post-immunization, and single-cell suspensions (at 2 × 105 cells/well) were plated into either IFN-γ- (a) or IL-17A- (b) coated 96-well filtration plates in the presence of MOG or medium alone. Following the overnight incubation, spots in the wells were analyzed using CTL ImmunoSpot Analyzer and the software. The data were presented as the mean ± SEM of each group, n = 3 mice, and performed in triplicate. Significant differences between hypoxia-exposed and normoxic EAE splenocytes are denoted with asterisks (*p < 0.05). IL-10 (c) and CXCL13 (d) levels in the spinal cord tissue homogenates were determined following the manufacturer’s instructions. Significant differences when compared to control mice are denoted with asterisks (*p < 0.05, **p < 0.01, ***p < 0.001), while the pound sign denotes the significant differences between hypoxia-exposed and normoxic EAE mice (## p < 0.01)

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