Fig. 2

Cx3cr1-GFP-positive microglia and Ccr2-RFP-positive macrophages in the retinas of Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice. a Retinal sections of Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice at 4 (upper panels), 6 (middle panels), and 8 weeks of age (lower panels) are shown. Cx3cr1-GFP is shown in green, and Ccr2-RFP is shown in red. GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer, IS inner segments, OS outer segments, RPE retinal pigment epithelium. The ONL boundaries are marked with broken lines. b Retinal sections of 8-week-old Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice are shown as a negative control. c The numbers of Cx3cr1-GFP, Ccr2-RFP, and Cx3cr1-GFP and Ccr2-RFP dual positive cells in 500 μm of the outer retina (from ONL to OS), and RPE of Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice were counted. Five different sections from different mice were used to count cell number. Asterisk indicates P < 0.05 vs 4-week-old Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice. n.d. not detected