Fig. 4

Analysis of retina and RPE flat mount. a Representative Cx3cr1-GFP (a), Ccr2-RFP (b), and Cx3cr1-GFP and Ccr2-RFP dual positive cells (amoeboid shape (c-1) and round shape (c-2)) observed in the retina and RPE flat mounts of 6-week-old Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice are shown. Cx3cr1-GFP and Ccr2-RFP merged images are shown. b, c The number of amoeboid- (a-c-1) or round-shaped (a-c-2) Cx3cr1-GFP and Ccr2-RFP dual positive cells observed in RPE flat mounts (1 mm²) of 6-week-old Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice was counted. Five different RPE flat mounts from different mice were used to count cell number. Asterisk indicates P < 0.05. b Bar graph shows the abundance of amoeboid- and round-shaped dual positive cells in RPE flat mounts. c Pie chart displays the ratios of Cx3cr1-GFP-positive, Ccr2-RFP-positive, and dual positive cells in the retina (upper) and RPE (lower)