Fig. 6

Flow cytometric analyses of WBC from Cx3cr1 ^GFP/GFP, Ccr2 ^RFP/RFP, and Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice and of the retinas from Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice. a WBC were harvested from Cx3cr1 ^GFP/GFP, Ccr2 ^RFP/RFP, and Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice. Cx3cr1-GFP and Ccr2-RFP are presented on the X and Y axes, respectively. Representative results from multiple flow cytometry analyses are shown. Boxes (a), (b), and (c) correspond to Ccr2-RFP, Ccr2-RFP, and Cx3cr1-GFP dual, and Cx3cr1-GFP-positive cells, respectively. b Neural retinas were obtained from 3-, 6-, and 8-week-old Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice and 6-week-old Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice. Cx3cr1-GFP and Ccr2-RFP are presented on the X and Y axes, respectively. Representative results from multiple flow cytometry analyses are shown. Boxes (a), (b), and (c) correspond to Ccr2-RFP, Ccr2-RFP, and Cx3cr1-GFP dual, and Cx3cr1-GFP-positive cells, respectively. Quantitative results are shown for each gate. Single asterisk indicates P < 0.05, double asterisks indicate P < 0.005, and triple asterisks indicate P < 0.001 vs 3-week-old Mertk ^−/− Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice. +/+ indicates 8-week-old Cx3cr1 ^GFP/+ Ccr2 ^RFP/+ mice