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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Suppression of MAPK attenuates neuronal cell death induced by activated glia-conditioned medium in alpha-synuclein overexpressing SH-SY5Y cells

Fig. 4

Blockage of p42/44 MAPK protects the SH-SY5Y cells from death caused by CM. a CM causes significant cell death after 24 h of incubation (*P < 0.05 vs NT and PD98059), which is decreased by PD98059 (# P < 0.05). Graph shows mean ± SEM of five independent experiments. The results are expressed as a percentage of maximal LDH release activity obtained after complete cell lysis. b Representative images of phosphorylated and total form of MAPK42/44 immunoblotting assay. c The densitometric analysis demonstrates that phosphorylation of MAPK42/44 is significantly higher in pHRIG cells treated with CM versus pHRIG + CM + PD98059. Two-way ANOVA followed with Bonferroni test: *P < 0.05 vs pHRIG + CM + PD98059 and A30P α-syn + CM. Graph shows mean ± SEM of four independent experiments

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