Skip to main content
Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: The nod-like receptor, Nlrp12, plays an anti-inflammatory role in experimental autoimmune encephalomyelitis

Fig. 8

IL-4 production by activated T cells from the WT or Nlrp12 −/− mice in vitro and in vivo. a CD4+ T cells were purified from the lymph nodes and spleens and stimulated with anti-CD3/CD28 antibodies. Intracellular production of IL-4 by activated CD4+ T cells was determined using flow cytometry. b The levels of IL-4 in serum samples from WT and Nlrp12 −/− EAE mice, measured by ELISA. c The level of IL-4 mRNA in lymph nodes from WT and Nlrp12 −/− EAE mice, quantified by real-time PCR. d, e The levels of IL-4 in tissue samples from the WT and Nlrp12 −/− EAE mice. Cerebellum and lymph node tissues were collected from the mice after 3 weeks of immunization with MOG:CFA. The tissues were homogenized in lysis buffer, and IL-4 levels were measured in tissue lysate by ELISA. Statistical analysis was done by Mann-Whitney U test, n = 3–6 per group. No significant difference was observed

Back to article page