Fig. 10

Ibuprofen dose-dependently inhibited the PGE2 production after LPS/IFNγ stimulation but did not affect the NO/ROS production in BV-2 cells. BV-2 cells were serum starved for 3 h followed by incubation with indicated concentrations of ibuprofen for 1 h before stimulated with a, c, e 200 ng/mL of LPS or b, d, f 20 ng/mL of IFNγ. a, b PGE2 production was measured in conditioned medium 16 h post-stimulation by the PGE2 ELISA assay as described in the text. c, d NO production was measured in conditioned medium 16 h post-stimulation by Griess protocol. e, f ROS production was measured 12 h post-stimulation by CM-H2DCFDA. Results were expressed as the mean ± SEM (n = 3) and significant difference between the respective groups was determined by one-way ANOVA followed by Dunnett’s post-tests, ***P < 0.001