Fig. 11

LOX inhibition mitigated NO or ROS production in BV-2 cells after LPS or IFNγ stimulation. BV-2 cells were serum starved for 3 h followed by incubation with indicated concentrations of NDGA for 1 h before stimulated with a, c 200 ng/mL LPS or b, d 20 ng/mL IFNγ. a, b NO production was measured in conditioned medium 16 h post-stimulation by Griess protocol. c, d ROS production was measured 12 h post-stimulation by CM-H2DCFDA. Results were expressed as the mean ± SEM (n = 3) and significant difference between the respective groups was determined by one-way ANOVA followed by Dunnett’s post-tests, *P < 0.05; **P < 0.01; ***P < 0.001