Fig. 2

Time course of protein expression in primary microglia and BV-2 cells after LPS/IFNγ stimulation. Primary microglial cells were stimulated with a 200 ng/mL LPS or b 20 ng/mL IFNγ. Cells were lysed, and proteins were collected and processed at indicated time post-stimulation. Western blot was performed to determine protein expression. Similarly, the same procedure was performed with BV-2 cells stimulated with c 200 ng/mL LPS or d 20 ng/mL IFNγ. Results are representative blots of two independent time course experiments for primary microglia and three experiments for BV-2 cells