Fig. 3

ERK1/2 contributed to cPLA₂ phosphorylation after LPS/IFNγ stimulation. BV-2 cells were starved for 3 h in serum-free DMEM. One hour prior to stimulation, cells were pretreated with indicated concentrations of MAPK inhibitors: U0126 (U) for ERK1/2 inhibition, SB202190 (SB) for p38 MAPK inhibition, and SP600125 (SP) for JNK inhibition. Cells were then stimulated with a, c 200 ng/mL LPS or b, d 20 ng/mL IFNγ. Cells were lysed and proteins were collected and processed 2 h after LPS stimulation or 8 h after IFNγ stimulation for Western blot analyses. a, b Representative blots. Protein expression was quantified with QuantityOne software for three separate experiments for c LPS-stimulated BV-2 cells and d IFNγ-stimulated BV-2 cells. Results were expressed as the mean ± SEM (n = 3) and significant difference from the respective group was determined by one-way ANOVA followed by Dunnett’s post-tests, *P < 0.05; **P < 0.01