Fig. 4

iNOS expression and NO production were significantly reduced in cPLA₂ KO primary microglia culture compared with WT culture. DIV5–7 primary microglia culture isolated from cPLA₂ KO or WT mice were stimulated with 200 ng/mL LPS or 20 ng/mL IFNγ for 24 h. Cells were then lysed, and proteins were collected/processed. a iNOS/cPLA₂/β-actin expressions were demonstrated by Western blot, and b, c iNOS/ β-actin levels were quantified with the QuantityOne software. Results were expressed as the mean ± SEM (n = 3), and significant difference between the respective paired groups was determined by t test, *P < 0.05; **P < 0.01. d Conditioned mediums from 48 h post-stimulation samples were collected for determination of nitrite concentration with the Griess protocol. Results were expressed as the mean ± SEM (n = 3) and significant difference between the respective groups was determined by t test, ***P < 0.001; ^^^P < 0.001