Fig. 5
Primary microglia from cPLA2 KO mice did not show a significant increase in ROS production by LPS and IFNγ. Different concentrations of a LPS or b IFNγ, as well as incubation time were used to evaluate the ROS production by primary microglia isolated from wild-type mice. Based on the results from WT culture, ROS production was evaluated at 48 h post-stimulation with different doses of c LPS or d IFNγ in microglial cells from cPLA2 KO mice. ROS production was quantified by CM-H2DCFDA fluorescence as described in the text. Results were expressed as the mean ± SEM (n = 3 WT LPS, n = 8 WT IFNγ, n = 3 KO LPS, n = 3 KO IFNγ), and significant difference between the groups was determined by one-way ANOVA followed by Dunnett’s post-tests, *P < 0.05; **P < 0.01; ***P < 0.001