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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Cytosolic phospholipase A2 plays a crucial role in ROS/NO signaling during microglial activation through the lipoxygenase pathway

Fig. 7

ROS production in BV-2 cells after LPS or IFNγ stimulation was inhibited by cPLA2 pharmacological inhibitors or siRNA knockdown. BV-2 cells were starved for 4 h in serum-free DMEM. One hour prior to stimulation, cells were pretreated with the indicated concentrations of cPLA2 inhibitors: a, b AACOCF3 or c, d pyrrophenone. Cells were then stimulated with a, c 200 ng/mL LPS or b, d 20 ng/mL IFNγ. Alternatively, BV-2 cells were transfected with siRNA against cPLA2 24 h before being stimulated with e LPS or f IFNγ. For all experiments, ROS production was measured 12 h post-stimulation by CM-H2DCFDA fluorescence as described in the text. Results were expressed as the mean ± SEM (n = 3) and significant difference between the respective groups was determined by one-way ANOVA followed by Dunnett’s post-tests, **P < 0.01, ***P < 0.001

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