Fig. 8
cPLA2 inhibition with AACOCF3 prevented morphological transformation of primary microglia associated with M1 activation. Immunocytochemical staining for Iba-1 and phalloidin was performed with DIV5 primary microglia culture with the following treatments for 24 h: a, b control; c, d 200 ng/mL LPS; e, f 20 ng/mL IFNγ; g, h 1 h of 5 μM AACOCF3 pretreatment with 200 ng/mL LPS for 24 h; i, j 1 h of 5 μM AACOCF3 pretreatment with 20 ng/mL IFNγ for 24 h. Iba-1 expression is represented by red fluorescent signal, phalloidin expression is represented by green fluorescent signal, and nuclear Hoechst staining is demonstrated in blue