Fig. 1

Cell body size correlates with the severity of status epilepticus. a, b Maximal intensity projection (MIP, z = 19 μm) images of microglial cells obtained in control mice (a) and in mice two days after kainate i.p. injection (b). The insets show higher magnification images. The line around cell bodies was drawn in a semi-automatic way to measure cell body size. The line on the process was based on the 3D image stack to avoid projection artifacts. Note the increase in cell body size of microglia and decrease of the longest process after SE. Scale bars 10 and 6 μm in the insets. c Soma size measurements obtained in all experiments classified according to the crisis level of animals, their median, and quartile value. d Cumulative probability of cell body size grouped according to the crisis level of animals, scored according to modified Racine’s scale. Note the progressive shift to the right with the increase of the severity of the induced SE. e Relationship between the median of cell body size for each animal and its crisis level or in control (ctrl, black). f Measurements of longest processes obtained in all experiments classified according to crisis level of animals, their median, and quartile value. g Cumulative probability of data represented in (f) grouped according to crisis level. Note the progressive shift to the left with increasing crisis severity. h Relationship between the median of the longest processes for each animal and its crisis level or in control (ctrl, black)