Fig. 6

XBD173 treatment prevents microglia reactivity and rescues cone photoreceptors in focal light-induced damage. a Light exposure regimen and mode of XBD173 administration. b Representative fundus image showing the focal light lesion 4 days after Cx3cr1 ^GFP/+ mice were exposed to 50,000 lux for 10 min. c–e Representative confocal images of GFP-positive retinal microglia in flat mounts from control mice (c) and lesion areas of light-exposed mice (d, e). The average size of microglial cells was assessed using the grid cross point system (f). Data show mean ± SEM (n = 40–180 cells per group) with ***p < 0.001. g–i Retinal flat mounts were processed for cone arrestin staining and examined by confocal microscopy. Representative confocal images of cone arrestin-positive cells in flat mounts from control mice (g) and lesion areas of light-exposed mice (h, i). j Average density of cone photoreceptor cells. Data show mean ± SEM (n = 6–8 retinas per group) with *p < 0.05; ***p < 0.001. Scale bar 50 μm