Fig. 3

IRAK4 is a direct target of miR-367 in microglia. a A IRAK4 3′UTR fragment containing wild-type or mutant miR-367-binding sites was cloned downstream of the luciferase reporter gene. The region of the IRAK4 mRNA 3′UTR predicted to be targeted by miR-367 as indicated. b Luciferase activity assays using reporters with wild-type or mutant IRAK4 3′UTRs were performed after cotransfection with miR-367 mimics or control in microglia. The luciferase activity of the control transfection in each experiment was used to normalize the data, and the luciferase activity of the control transfection was set equal to 1. Experiments performed in triplicate showed consistent results. Data are presented as the mean ± SD of three independent experiments. *P < 0.05