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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Self-reactive CD4+ T cells activated during viral-induced demyelination do not prevent clinical recovery

Fig. 1

Peripheral activation and CNS recruitment of self-reactive CD4+ T cells is myelin driven. a Irradiated Wt mice received 1 × 106 naïve MOG-specific 2D2 (CD90.1+) CD4+ T cells i.v. Two weeks post-transfer and prior infection, CD45hi cells within the CNS were analyzed by flow cytometry and compared to age-matched non-irradiated Wt mice. b Representative FACS density plots of 2D2 cells within CD4+ T cells isolated from the CNS and CLN at days 0, 7, 14, 21, and 30 p.i. c Frequency and absolute number of 2D2 cells within CD4+ T cells in the CNS and CLN at days 0, 7, 14, 21, and 30 p.i. Data represent the mean of three individual mice per time point. d Frequencies of proliferating host CD90.1CD4+ and transferred CD90.1+CD4+ T cells within the CNS and CLN, characterized by BrdU incorporation and analyzed by flow cytometry. Data represent the mean ± SEM of three individual mice. e Mean clinical scores following JHMV infection of WT mice without transfer (n = 15) or following 2D2 T cell transfer (n = 32). f Irradiated Wt mice received 1 × 106 MOG-specific 2D2 (CD90.1+) CD4+ T cells and an equal number of OVA-specific OT-II (Ly5.1+) CD4+ T cells. Percentages of CD90.1+ 2D2 and Ly5.1+ OT-II cells within the CLN before infection (d0) and in both the CLN and CNS at day 14 p.i. Bar graphs represent the mean ± SEM of two separate experiments

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