Fig. 2

CLN-derived CD11b⁺ cells activate MOG-specific CD4⁺ T cells. a Percentages of CD11b⁺ cells and CD11c⁺ cells within the CD11b⁺ population in the CLN from days 7 to 30 p.i. b Representative CFSE dilution gated on 2D2 CD90.1⁺CD4⁺ T cells 4 days after co-culture with media only (No APC), CLN-derived CD11b⁻, and CD11b⁺ cells at day 14 p.i. c Percentages of proliferating 2D2 cells co-cultured with media only (No APC), CD11b⁻, and CD11b⁺ cells isolated from the CLN of JHMV-infected mice at days 7, 10, 14, 21, and 30 p.i. Data represent the mean ± SEM with n = 3–12 pooled mice per time point from three separate experiments. #p < 0.05 and ###p < 0.001 depict significant differences between No APC and CD11b⁺ cells, whereas §§§p < 0.001 depicts significant differences between CD11b⁻ and CD11b⁺ cells determined by a two-way ANOVA with Bonferroni post-test. Significant differences between time points are indicated by **p < 0.01 and ***p < 0.001 using Dunn’s multiple comparison test