Fig. 4

MHC class II expression and exogenous myelin antigen presentation by CNS-infiltrating CD11b⁺ cells and microglia. a Percentage and mean fluorescence intensity (MFI) expression of MHC class II by CNS-derived control (CD45^hiCD11b⁻), infiltrating CD11b⁺ (CD45^hiCD11b⁺), and microglia (CD45^lowCD11b⁺) cells between days 7 and 30 p.i. Data represent the average ± SEM of three separate experiments with n = 3 pooled mice per experiment. Histogram of MHC class II expression by CD45^hiCD11b⁻ (black line), infiltrating CD11b⁺ (red line), and microglia (blue line) during JHMV infection. b MFI expression of CD80 and CD86 by CNS-infiltrating CD11b⁺ (CD45^hiCD11b⁺) and microglia (CD45^lowCD11b⁺) cells between days 7 and 30 p.i. Data represent the average ± SEM of 3 separate experiments with n = 3 pooled mice per experiment. c Proliferation of 2D2 CD4⁺ T cells co-cultured with CD45^hiCD11b⁺ and CD45^lowCD11b⁺ cells with or without exogenously added MOG^35-55 peptide