Fig. 6
Myelin-specific CD4+ T cell activation following JHMV infection. a Number of virus-specific M133, MOG35-55-specific, and MBP60-80-specific CD4+ T cells per 106 cells in the CLN and CNS of JHMV-infected mice between days 7 to 60 p.i. Each dot represents the triplicates of individual experiments at various dilutions of at least eight pooled mice. Dashed lines represent the limit of detection determined as frequencies of IFN-γ-secreting cells specific for M133, MOG35-55, and MBP60-80 detected in naïve animals. Red bars represent the mean ± SEM. Significant differences between time points are indicated *p < 0.05, **p < 0.01, and ***p < 0.001. Significant differences compared to baseline levels are indicated #p < 0.05, ##p < 0.01, and ###p < 0.001 using unpaired t test. b Percentage of mice exhibiting IFN-γ-secreting MOG and MBP-specific CD4+ T cells above maximal baseline levels in the CLN and CNS between days 7 and 60 p.i. c Clinical symptoms following JHMV infection. Data represent the mean ± SEM of 32 mice. d Correlation between frequencies of SR T cells at days 45 and 60 p.i. with average clinical scores, coefficient of determination R 2 = 0.1417