Fig. 2
Lack of IFN-β expression does not affect cerebral Gal-3 immunoreactivity within the first 8 days after tMCAo. a Top panel representative composite micrograph of Gal-3 (Alexa488, green) and Iba-1 (Cy3, red) immunoreactivities within the injured cerebral hemisphere 8 days after tMCAo (WT mouse). IC infarct core, PI peri-infarct region. Bottom panel corresponding micrograph denoting Gal-3 and Iba-1 colocalization within the infarct core; note that colocalization is partial. Scale bars, 500 and 20 μm, respectively. b Percentage of Iba-1 colocalizing with Gal-3 (87.4 ± 0.05 % for WT mice) and c Gal-3 colocalizing with Iba-1 (96.5 ± 0.07 % for WT mice). c–h Area (μm2), perimeter (μm), circularity (0–1), Feret’s diameter (μm), and aspect ratio of Gal-3+ cells within infarct core, respectively. i Gal-3 immunoreactivity (I/pixel). a–i WT, n = 6–7; IFN-βKO: n = 7–8