Fig. 1

Upregulation of Sur1 in astrocytes in EAE. a–c White matter of lumbar spinal cord sections from control (CTR) (a) or WT/EAE mice on pid-10 or pid-30, as indicated (b, c), immunolabeled for Sur1; all imaging parameters were identical for the three panels; nuclei stained with DAPI. d Lumbar spinal cord section from a pid-30 WT/EAE mouse immunolabeled for Sur1 (green; left panel) and co-labeled for GFAP (red; middle panel) to identify astrocytes; merged images are also shown (d, right panel). e Box plots showing the percent of white matter with Sur1 immunopositivity under control conditions (pid-0) and at pid-10 and pid-30; five mice/group. f Bar graph showing Pearson’s correlation coefficient for Sur1 co-localization with GFAP, Iba1, and MBP; five mice/group. g, h Gray matter of lumbar spinal cord section from a pid-30 WT/EAE mouse double-labeled for Sur1 (red) and co-labeled for NeuN (green) to identify neurons; merged images are shown. The results illustrated are representative of findings in five mice/group; scale bars, 100 μm (a–c); 50 μm (d, g, h)