Table 1 The PCR conditions for IL-1β, TNF-α, IL-6, COX-2, iNOS, CRH, GAPDH, and 16S rDNA
Gene | Primer sequences | Working primer concentration (μM) | Annealing temperature (°C) | Extension time (s) | Amplicon size (base pair) |
|---|---|---|---|---|---|
IL-1β (118130747) | F: 5′-CCTCCTTGCCTCTGATGG-3′ | 0.4 | 60 | 10 | 99 |
R: 5′-AGTGCTGCCTAATGTCCC-3′ | |||||
TNF-α (518831586) | F: 5′-CCCCAGTCTGTATCCTTCT-3′ | 0.4 | 59 | 30 | 106 |
R: 5′-ACTGTCCCAGCATCTTGT-3′ | |||||
IL-6 (13624310) | F: 5′-GGCAATTCTGATTGTATG-3′ | 0.4 | 56 | 30 | 208 |
R: 5′-CTCTGGCTTTGTCTTTCT-3′ | |||||
COX-2 (118130137) | F: 5′-GATGACTGCCCAACTCCC-3′ | 0.25 | 60 | 10 | 191 |
R: 5′-AACCCAGGTCCTCGCTTA-3′ | |||||
iNOS (146134510) | F: 5′-AAACGCTTCACTTCCAATG-3′ | 0.4 | 61 | 30 | 296 |
R: 5′-CAATCCACAACTCGCTCC-3′ | |||||
CRH (292781723) | F: 5′-GTGCGGGCTCACCTACCAA-3′ | 0.4 | 57 | 10 | 107 |
R: 5′-AGGCAGGCAGGACGACAGA-3′ | |||||
GAPDH (576080554) | F: 5′-ATTCAACGGCACAGTCAA-3′ | 0.4 | 56 | 10 | 77 |
R: 5′-CTCGCTCCTGGAAGATGG-3′ | |||||
16S rDNA (J01859.1) | F: 5′-GCAAGCGGACCTCATAAA-3′ | 0.25 | 54 | 20 | 100 |
R: 5′-ATTCACCGTGGCATTCTG-3′ |