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Table 1 The PCR conditions for IL-1β, TNF-α, IL-6, COX-2, iNOS, CRH, GAPDH, and 16S rDNA

From: PKR deficiency alters E. coli-induced sickness behaviors but does not exacerbate neuroimmune responses or bacterial load

Gene Primer sequences Working primer concentration (μM) Annealing temperature (°C) Extension time (s) Amplicon size (base pair)
IL-1β (118130747) F: 5′-CCTCCTTGCCTCTGATGG-3′ 0.4 60 10 99
R: 5′-AGTGCTGCCTAATGTCCC-3′
TNF-α (518831586) F: 5′-CCCCAGTCTGTATCCTTCT-3′ 0.4 59 30 106
R: 5′-ACTGTCCCAGCATCTTGT-3′
IL-6 (13624310) F: 5′-GGCAATTCTGATTGTATG-3′ 0.4 56 30 208
R: 5′-CTCTGGCTTTGTCTTTCT-3′
COX-2 (118130137) F: 5′-GATGACTGCCCAACTCCC-3′ 0.25 60 10 191
R: 5′-AACCCAGGTCCTCGCTTA-3′
iNOS (146134510) F: 5′-AAACGCTTCACTTCCAATG-3′ 0.4 61 30 296
R: 5′-CAATCCACAACTCGCTCC-3′
CRH (292781723) F: 5′-GTGCGGGCTCACCTACCAA-3′ 0.4 57 10 107
R: 5′-AGGCAGGCAGGACGACAGA-3′
GAPDH (576080554) F: 5′-ATTCAACGGCACAGTCAA-3′ 0.4 56 10 77
R: 5′-CTCGCTCCTGGAAGATGG-3′
16S rDNA (J01859.1) F: 5′-GCAAGCGGACCTCATAAA-3′ 0.25 54 20 100
R: 5′-ATTCACCGTGGCATTCTG-3′
  1. The real-time PCR conditions for IL-1β, TNF-α, IL-6, COX-2, iNOS, CRH, GAPDH, and 16S rDNA. Accession numbers from the Genebank are shown in brackets.
  2. COX-2 cyclooxygenase-2, CRH corticotrophin-releasing hormone, iNOS inducible nitric oxide synthase, TNF-α tumor necrosis factor-α, IL interleukin