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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Amelioration of Japanese encephalitis by blockage of 4-1BB signaling is coupled to divergent enhancement of type I/II IFN responses and Ly-6Chi monocyte differentiation

Fig. 3

Impaired JEV-specific T cell immunity in 4-1BB signal-ablated mice. a, b JEV-specific CD4+ T cell responses. c JEV-specific CD8+ T cell responses. Splenocytes prepared from surviving mice at 7 or 14 dpi were stimulated with JEV epitope peptide of CD4+ T cells (a NS1132–145; b NS3563-574) or CD8+ T cells (NS4B215–223) for 12 or 6 h, respectively. The frequency and absolute number of JEV-specific CD4+ and CD8+ T cells were determined by intracellular CD154 and cytokine (IFN-γ and TNF-α) staining, combined with surface CD4 and CD8 staining. Values in representative dot-plots denote the average percentage of the indicated cell population, and bar charts show the average ± SD of values derived from at least four mice per group. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the levels of the indicated groups

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