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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Amelioration of Japanese encephalitis by blockage of 4-1BB signaling is coupled to divergent enhancement of type I/II IFN responses and Ly-6Chi monocyte differentiation

Fig. 4

A skewed response of 4-1BB signal-ablated mice to IFN-γ-producing CD4+ Th1. a The frequency and number of CD4+CD25+Foxp3+ Tregs. The frequency and total number of CD4+CD25+Foxp3+ Treg cells in the spleen of WT and 4-1BB KO mice were determined by flow cytometric analysis 3 and 5 dpi. Dot-plots show the frequency of CD25+Foxp3+ Tregs after gating on CD4+ T cells. b The frequency and number of IFN-γ+CD4+ Th1 and IL-17+CD4+ Th17 cells. The frequency and number of IFN-γ+CD4+ Th1 and IL-17+CD4+ Th17 cells were determined by intracellular cytokine staining in response to PMA + ionomycin stimulation of splenocytes prepared from WT and 4-1BB KO mice 3 and 5 dpi. Values in the representative dot-plots denote the average percentage of the indicated cell populations, and bar graphs show the average ± SD of values derived from at least four mice per group. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the levels of the indicated groups

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