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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: SDF1–CXCR4 signaling contributes to persistent pain and hypersensitivity via regulating excitability of primary nociceptive neurons: involvement of ERK-dependent Nav1.8 up-regulation

Fig. 4

Blockade of BV-induced phosphorylation of ERK and up-regulation of Nav1.8 protein expression in the lumbar DRG by peripherally local injection of AMD3100. a Representative immunofluorescent photomicrographs showing expression of Nav1.8 in the lumbar DRG from saline-treated rats, BV-treated rats, and rats receiving intraplantar AMD3100 or intrathecal U0126 10 min before intraplantar BV injection. b Quantification of the mean immufluorescent intensity of Nav1.8 showing an increase in expression following intraplantar (i.pl.) BV injection, and a reduction in rats receiving AMD3100 or U0126 administration 10 min prior to BV (n = 5/group, ***P <0.001, ##P < 0.001, ††P < 0.001 vs. saline). c Western blot showing that AMD3100 prevented the BV-induced up-regulation of Nav1.8 and pERK activation in lumbar DRG from occurring (n = 3/group, ***P < 0.001 vs. saline; #P < 0.05, ###P < 0.001 vs. BV). d Western blot showing that intrathecal U0126 prevented the BV-induced up-regulation of Nav1.8 and pERK activation in lumbar DRG from occurring (n = 3/group, ***P < 0.001 vs. saline; #P < 0.05 vs. BV). e Western blot showing that intraplantar U0126 prevented the BV-induced up-regulation of Nav1.8 and pERK activation in lumbar DRG from occurring (n = 3/group, **P < 0.01, ***P < 0.001 vs. saline; #P < 0.05, ###P < 0.001 vs. BV)

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