Fig. 2

Quantification of cavity volume and inflammatory reaction after TBI. Serial sections were stained for GFP (or Iba1) to visualize microglia or RFP to visualize infiltrating bone marrow-derived monocytes (BMDM). Whole slices, lesion cavity, and area of the slices occupied by monocytes (RFP⁺ cells) or microglia (increased GFP or Iba1 immunoreactivity) were manually outlined to calculate their area; all sections were summed up to calculate volumes. Pathology was quantified as a percentage of the analyzed brain tissue (4 mm total thickness) that contained the lesion cavity (a), infiltrating monocytes (b), or increased microglial staining (c). Statistics: two-way ANOVA and Tukey’s post hoc test. Comparisons between groups are shown with horizontal lines; vertical line in figure legend indicates main effect of genotype. *p < 0.05; **p < 0.01; ***p < 0.001