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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Trazodone regulates neurotrophic/growth factors, mitogen-activated protein kinases and lactate release in human primary astrocytes

Fig. 5

Expression of pro-inflammatory genes, trophic and transcription factors in an experimental model of inflammation. a Human astrocytes were treated with 50 μg/ml LPS and 50 ng/ml TNF-α for 24 h. b, c Human astrocytes were treated with medium alone (control), different concentrations of TDZ (100 nM-1 μM), or FLUOX (10 μM) for 24 h (b) or 72 h (c); after drug removal, cells were incubated with 50 μg/ml LPS and 50 ng/ml TNF-α for an additional 24 h. At the end of treatment, total RNA was extracted, and relative mRNA quantification of NF-kB, CREB, mTOR and BDNF was performed by RT-PCR. The data are expressed as fold changes vs. control and represent the mean ± SEM of three different experiments, each performed in duplicate. Statistical significance was determined using a one-way ANOVA-Tukey HSD post hoc test: *P < 0.05, **P < 0.01, ***P < 0.001 vs. control; # P < 0.05, ##P < 0.01, ### P < 0.001 vs. cells treated with LPS-TNF-α

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