Trazodone regulates neurotrophic/growth factors, mitogen-activated protein kinases and lactate release in human primary astrocytes

Table 1 Nucleotide sequences, annealing temperature and product size of the primers utilised in PCR experiments

Gene	Primer nucleotide sequences	Product size (base pairs)	Annealing temperature
CREB	FOR: 5’-AAGCTGAAAGTCAACAAATGACA-3’	240	52 °C
CREB	REV: 5’-CCTCTTTTCAGAAAAATTCAGGA-3’	240	52 °C
BDNF	FOR: 5’-TACATTTGTATGTTGTGAAGATGTTT-3’	131	56 °C
BDNF	REV: 5’- TTACTCGCCCCGGACCCTCT-3’	131	56 °C
NF-kB	FOR: 5’- GCTCCGGAGACCCCTTCCA-3’	198	54 °C
NF-kB	REV: 5’- GGTTTGAGGTAGTTTCCCAGT-3’	198	54 °C
mTOR	FOR: 5’-CCGTTCCATCTCCTTGTCACG-3’	209	56 °C
mTOR	REV: 5’-CCACTTACTCTGCAGTGTG-3’	209	56 °C
β-actin	FOR: 5’-GCACTCTTCCAGCCTTCCTTCC-3’	254	55 °C
β-actin	REV-5’-GAGCCGCCGATCCACACG-3’	254	55 °C

ISSN: 1742-2094