Skip to main content
Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1

Fig. 2

TNFα does not induce detectable DNA strand breaks. a PANT staining shows no detectable increase in DNA strand breaks in either microglia or astrocyte cultures after 60-min incubation with 15 ng/ml TNFα. The DNA alkylating agent, MNNG (10 μM) is used as a positive control. Scale bar, 50 μm. b Quantification of PANT positive cells (* p < 0.05, n = 3). c Immunostaining for PAR shows comparable PAR accumulation in cells incubated for 60 min with 15 ng/ml TNFα or 10 μM MNNG. Scale bar, 25 μm

Back to article page