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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1

Fig. 4

PLC activation triggers microglial morphologial transformation and activation of PARP and NF-κB, independent of TNFα. a The PLC activator m-3M3FBS (25 μM, 60 min) induces microglial morphological transformation and PARP activation. The PC-PLC inhibitor, D609 (10 μM) prevents both effects of the PLC activator. Scale bar, 10 μm. b Quantification of PAR positive cells (*p < 0.05, n = 3). c NF-κB transcriptional activity was evaluated in cultures transfected with a reporter gene in which dEGFP expression (green) is driven by NF-κB p65 subunit binding. The PLC activator m-3M3FBS (25 μM, 60 min) induced NF-κB activation as seen by dEGFP expression. This NF-κB transcriptional activity was blocked by both PLC inhibitor D609 (10 μM) and PARP-1 inhibitor DPQ (25 μM)

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