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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1

Fig. 8

PARP-1 is not required for NF-κB nuclear translocation. The subcellular localization of the NF-κB p65 subunit (green) was visualized by immunostaining in wt and PARP-1−/− astrocyte cultures after TNFα exposure. Nuclei were stained with propidium iodide (red). Cells fixed after 30 min of TNFα (15 ng/ml) exposure show overlap of these signals (yellow), indicative of p65 movement into the nucleus. This movement was not blocked by the PARP inhibitor DPQ (25 μM) or in PARP-1−/− cells. Representative of n = 3. Scale bar, 50 μm

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