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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1

Fig. 9

PLC is not required for DNA damage-induced PARP-1 activation. a Astrocytes were immunostaining for PAR (green) and nuclei were counterstained with propidium iodide (red). PAR formation induced by MNNG (10 μM, 60 min) was prevented by inhibitors of PARP (DPQ; 25 μM) and MEK-ERK1/2 (PD98059; 10 μM), but not by PC-PLC inhibitor (D609; 10 μM) or by calcium-free medium (500 μM EGTA). Intracellular calcium chelation with BAPTA-AM (2 μM) produced a partial reduction in PAR formation. Scale bar, 40 μm. b Quantification of PAR positive cells (# p < 0.05 compared to control, *p < 0.05 compared to MNNG alone, n = 3)

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