Table 1 Microglial cytokine and trophic factor release in vitro upon TNFα stimulation

IFNγ

IL-1β

KC

MCP-1

MIP-1β

Ctrl (wt)

221.97 ± 36.69

20.4 ± 1.9

157.12 ± 20.8

103.63 ± 21.5

2840.02 ± 357.4

TNFα (wt)

335.76 ± 21.3^*

59.38 ± 2.8^*

408.13 ± 60.0^*

668.0 ± 78.7^*

4777.28 ± 527.6^*

TNFα + PJ34 (wt)

284.09 ± 21.6^**

35.2 ± 2.3^**

197.75 ± 25.4^**

339.35 ± 77.9^**

3081.38 ± 353.7^**

PJ34 (wt)

68.48 ± 16.6^*

1.35 ± 2.0^*

24.67 ± 11.1^*

153.96 ± 33.4

1664.42 ± 361.8^*

Ctrl (PARP-1^−/−)

101.03 ± 21.2^*

13.82 ± 4.0^*

22.30 ± 6.7^*

46.84 ± 16.7^*

1104.99 ± 172.0^*

TNFα (PARP-1^−/−)

101.97 ± 17.9^**

14.12 ± 3.0^**

67.07 ± 18.9^**

84.10 ± 28.7^**

1118.34 ± 146.1^**

IL-4

IL-10

IL-13

TGF-β

VEGF

Ctrl (wt)

0.8 ± 0.5

21.1 ± 1.2

1.03 ± 0.1

1.11 ± 0.2

4.280 ± 1.2

TNFα (wt)

1.46 ± 0.5

22.78 ± 10.9

0.2 ± 0.3^*

1.35 ± 0.2

2.82 ± 0.6

TNFα + PJ34 (wt)

6.09 ± 0.8^*

34.77 ± 8.3^*

25.00 ± 4.8^**

1.41 ± 0.1

39.57 ± 6.6^**

PJ34 (wt)

1.48 ± 0.4

19.0 ± 1.3

0.89 ± 0.2

1.31 ± 0.1^*

22.92 ± 3.7^*

Ctrl (PARP-1^−/−)

0.77 ± 0.1

7.47 ± 11.3

5.73 ± 1.1^*

1.37 ± 0.1^*

13.63 ± 5.3^*

TNFα (PARP-1^−/−)

0.86 ± 0.4

11.16 ± 1.9

2.69 ± 0.5^**

1.90 ± 0.2^**

21.98 ± 4.7^**