| |
Pro-inflammatory cytokines
|
| |
IFNγ
|
IL-1β
|
KC
|
MCP-1
|
MIP-1β
|
|
Ctrl (wt)
|
221.97 ± 36.69
|
20.4 ± 1.9
|
157.12 ± 20.8
|
103.63 ± 21.5
|
2840.02 ± 357.4
|
|
TNFα (wt)
|
335.76 ± 21.3*
|
59.38 ± 2.8*
|
408.13 ± 60.0*
|
668.0 ± 78.7*
|
4777.28 ± 527.6*
|
|
TNFα + PJ34 (wt)
|
284.09 ± 21.6**
|
35.2 ± 2.3**
|
197.75 ± 25.4**
|
339.35 ± 77.9**
|
3081.38 ± 353.7**
|
|
PJ34 (wt)
|
68.48 ± 16.6*
|
1.35 ± 2.0*
|
24.67 ± 11.1*
|
153.96 ± 33.4
|
1664.42 ± 361.8*
|
|
Ctrl (PARP-1−/−)
|
101.03 ± 21.2*
|
13.82 ± 4.0*
|
22.30 ± 6.7*
|
46.84 ± 16.7*
|
1104.99 ± 172.0*
|
|
TNFα (PARP-1−/−)
|
101.97 ± 17.9**
|
14.12 ± 3.0**
|
67.07 ± 18.9**
|
84.10 ± 28.7**
|
1118.34 ± 146.1**
|
| |
Anti-inflammatory cytokines and trophic factors
|
| |
IL-4
|
IL-10
|
IL-13
|
TGF-β
|
VEGF
|
|
Ctrl (wt)
|
0.8 ± 0.5
|
21.1 ± 1.2
|
1.03 ± 0.1
|
1.11 ± 0.2
|
4.280 ± 1.2
|
|
TNFα (wt)
|
1.46 ± 0.5
|
22.78 ± 10.9
|
0.2 ± 0.3*
|
1.35 ± 0.2
|
2.82 ± 0.6
|
|
TNFα + PJ34 (wt)
|
6.09 ± 0.8*
|
34.77 ± 8.3*
|
25.00 ± 4.8**
|
1.41 ± 0.1
|
39.57 ± 6.6**
|
|
PJ34 (wt)
|
1.48 ± 0.4
|
19.0 ± 1.3
|
0.89 ± 0.2
|
1.31 ± 0.1*
|
22.92 ± 3.7*
|
|
Ctrl (PARP-1−/−)
|
0.77 ± 0.1
|
7.47 ± 11.3
|
5.73 ± 1.1*
|
1.37 ± 0.1*
|
13.63 ± 5.3*
|
|
TNFα (PARP-1−/−)
|
0.86 ± 0.4
|
11.16 ± 1.9
|
2.69 ± 0.5**
|
1.90 ± 0.2**
|
21.98 ± 4.7**
|
- Data is presented as a pg of cytokine detected from the cell culture medium, normalized to mg of protein in corresponding cell culture well (mean ± stdev). PJ34 is a PARP-1 inhibitor. *
p ≤ 0.05 compared to ctrl (wt), **
p ≤ 0.05 compared to TNFα (wt), n = 3–4
