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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Leucine-rich repeat kinase 2 positively regulates inflammation and down-regulates NF-κB p50 signaling in cultured microglia cells

Fig. 2

Lrrk2 / primary microglia cells exhibit attenuated inflammation. a Lrrk2 +/+ and Lrrk2 / primary microglia culture purity. Representative images of Lrrk2 +/+ and Lrrk2 / primary microglia culture stained for microglial CD11b (green), astrocytic GFAP (red), and nuclei with Hoechst (blue). Scale bar 25 μm. b Lrrk2 +/+ and Lrrk2 / microglia lysates treated with LPS or PBS as control were subjected to immunoblotting using LRRK2 and β-tubulin antibodies. c Quantification of LRRK2 protein is normalized for β-tubulin. Data are representative of three independent experiments (bars represent the mean ± SEM; unpaired t test). d Lrrk2 +/+ and Lrrk2 / microglia lysates treated with LPS or PBS as control were subjected to semi-quantitative PCR. e Quantification of LPS-mediated IL-1β mRNA is normalized for GAPDH. Data are representative of three independent experiments (bars represent the mean ± SEM; unpaired t test; *p < 0.05). f Lrrk2 +/+ and Lrrk2 / microglia lysates treated with LPS or PBS as control were subjected to immunoblotting using COX-2 and IL-1β antibodies. g Quantification of LPS-mediated COX-2 and IL-1β are normalized for β-tubulin. Data are representative of three independent experiments (bars represent the mean ± SEM; unpaired t test; *p < 0.05, **p < 0.01)

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