Fig. 3

Lrrk2 ^−/− primary microglia cells exhibit attenuated inflammation after α-syn fibrils priming. a After purification, α-syn was subjected to reverse phase HPLC chromatography. α-syn aggregation and fibrils formation were validated by Thioflavin assay (b) and TEM imaging (c). Scale bar 100 nm. d Lrrk2 ^+/+ microglia lysates treated with 25 μM α-syn fibrils (concentration before fibrillation) or 25 nM α-syn monomer as control for 6 and 20 h were subjected to immunoblotting using IL-1β antibody. e Lrrk2 ^+/+ and Lrrk2 ^−/− microglia lysates treated with 25 μM α-syn fibrils (concentration before fibrillation) or 25 nM α-syn monomer as control were subjected to immunoblotting using IL-1β antibody. f Quantification of α-syn fibrils-mediated IL-1β expression is normalized for β-tubulin. Data are representative of four independent experiments (bars represent the mean ± SEM; unpaired t test; **p < 0.01). g Lrrk2 ^+/+ microglia lysates treated with 25 μM α-syn fibrils (concentration before fibrillation) or 25 nM α-syn monomer as control for 20 h were subjected to immunoblotting using LRRK2 antibody. h Quantification of LRRK2 expression is normalized for β-tubulin. Data are representative of three independent experiments (bars represent the mean ± SEM)