Fig. 4

LRRK2 does not influence p65 phosphorylation and p50 maturation upon LPS priming. a Lrrk2 ^+/+ and Lrrk2 ^−/− microglia lysates treated with LPS or PBS as control were subjected to immunoblotting using NF-κB P-p65 and p65 antibodies. b Quantification of P-p65 is normalized for p65 total protein. Data are representative of three independent experiments (bars represent the mean ± SEM; one-way ANOVA comparing all groups, Tukey’s multiple comparison post-hoc test). c Lrrk2 ^+/+ and Lrrk2 ^−/− microglia lysates treated with LPS or PBS as control were subjected to immunoblotting using NF-κB p105/p50 antibody. Quantification of p105 (d) and p50 (e) proteins are normalized for GAPDH. Data are representative of three independent experiments, respectively, (bars represent the mean ± SEM; one-way ANOVA comparing all groups, Tukey’s multiple comparison post hoc test)