Fig. 8

TLR agonists regulate TLR2, TLR3 and TLR4 gene expression in rat cortical astrocytes. Enriched or purified (L-LME-treated) astrocytes were challenged with LPS (100 ng/ml), zymosan (10 μg/ml) or poly(I:C) (50 μg/ml) and processed after 6 h for TLR2, TLR3 and TLR4 mRNA expression by RT-PCR. Data are means ± SEM (n = 3) normalized to GAPDH levels and are representative of three experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (‘Ctr’). ^§§ p < 0.01 and ^§§§ p < 0.001 vs L-LME-treated cells. Qualitatively similar results were obtained after 24 h, although relative mRNA levels were lower