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Table 1 Primer pairs used in this study

From: Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes

Gene target Primer name Sequence
GAPDH GAPDH For 5′-CAAGGTCATCCATGACAACTTTG-3′
GAPDH Rev 5′-GGGCCATCCACAGTCTTCTG-3′
IL-1ß IL-1ß For 5′-TGTGGCAGCTACCTATGTCT-3′
IL-1ß Rev 5′-GGGAACATCACACACTAGCA-3′
TNF-α TNF-a For 5′-CATCTTCTCAAAACTCGAGTGACAA-3′
TNF-a Rev 5′-TGGGAGTAGATAAGGTACAGCCC-3′
IL-6 IL-6 For 5′-TCACAGAAGGAGTGGCTAAGG-3′
IL-6 Rev 5′-GCTTAGGCATAGCACACTAGG-3′
TLR2 TLR2 For 5′-TCCATGTCCTGGTTGACTGG-3′
TLR2 Rev 5′-AGGAGAAGGGCACAGCAGAC-3′
TLR4 TLR4 For 5′-GATTGCTCAGACATGGCAGTTTC-3′
TLR4 Rev 5′-CACTCGAGGTAGGTGTTTCTGCTAA-3′
TLR3 TLR3 For 5′-TGAAAACTACGGCGATGCAG-3′
TLR3 Rev 5′-AGGCAGTTTTACTTCCCCGA-3′