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Table 3 TLR agonists up-regulate IL-1β intracellular content at 6 and 24 h in purified rat cortical microglia and enriched, but not microglia-depleted, astrocytes

From: Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes

Treatment Microglia (pg IL-1β/well) Astrocytes (pg IL-1β/well)
   - - - L-LME - - - L-LME
6 h 24 h 6 h 6 h 24 h 24 h
Control 16.0 ± 1.0 0 62.6 ± 4.1 37.6 ± 1.3 66.4 ± 2.0 41.5 ± 2.0
LPS 1711.7 ± 63.9 878.1 ± 30.2 5150.4 ± 162.4 154.4 ± 26.7 6636.9 ± 285.5 155.0 ± 17.6
Zymosan 2036.2 ± 53.9 1382.6 ± 29.1 6910.8 ± 742.0 186.5 ± 15.8 7081.9 ± 405.1 157.3 ± 15.2
Poly(I:C) 1710.0 ± 125.1 631.1 ± 41.7 3911.5 ± 96.8 138.5 ± 4.3 3174.6 ± 151.7 62.6 ± 0.7
  1. Purified microglia and enriched or purified (L-LME-treated) astrocytes were challenged with LPS (100 ng/ml), zymosan (10 μg/ml) or poly(I:C) (50 μg/ml) and processed after 6 and 24 h for intracellular IL-1β content by ELISA. Data are means ± SEM (n = 3). Note the lack of astrocyte responsiveness after L-LME. Values for IL-1β content of the corresponding culture media samples were <5 % of the intracellular values (not shown)